SOP templatesHealthcare

Infection Control SOP Template

A ready-to-use SOP for applying transmission-based precautions, donning and doffing personal protective equipment, and responding to exposures.

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Ilia PirozhenkoReviewed by Ilia Pirozhenko, Founder, Perfect WikiUpdated September 15, 202612 steps4 roles3 min read
Standard operating procedureSOP-HLC-003 ยท Rev 1.0
Owner
Infection Prevention Coordinator
Effective date
September 15, 2026
Review cycle
Every 12 months

1.Purpose

To prevent the spread of infectious disease between patients and staff by applying the correct precautions, personal protective equipment and cleaning at every stage of care.

2.Scope

Applies to all clinical areas where patients may require standard, contact, droplet or airborne precautions. Sterile processing and surgical asepsis are covered by separate SOPs.

Definitions

Standard precautions
Baseline infection prevention practices used for every patient regardless of diagnosis, including hand hygiene and PPE based on anticipated exposure.
Transmission-based precautions
Additional precautions, such as contact, droplet or airborne, added for patients known or suspected to have a specific infectious organism.
Donning and doffing
Putting on and taking off personal protective equipment in a sequence that avoids self-contamination.
Exposure
Contact with blood, body fluids or an infectious organism that may require follow-up evaluation.

3.Responsibilities

Clinical Staff Member
Applies the correct precautions, dons and doffs PPE correctly, and reports possible exposures immediately.
Charge Nurse
Confirms precaution signage, assigns isolation rooms and verifies PPE supply on the unit.
Infection Prevention Coordinator
Determines precaution type, investigates exposures and clusters, and updates precaution orders.
Environmental Services Technician
Cleans and disinfects isolation rooms and restocks PPE stations outside precaution rooms.

RACI matrix

ActivityClinical Staff MemberCharge NurseInfection Prevention CoordinatorEnvironmental Services Technician
Determine and order precaution typeICR/A-
Post signage and assign isolation roomIR/ACI
Don and doff PPE correctlyR/AIIR/A
Report and follow up on exposuresRCR/A-

R = Responsible, A = Accountable, C = Consulted, I = Informed

4.Materials and PPE

Materials, tools and systems

  • โ†’Precaution signage (contact, droplet, airborne)
  • โ†’PPE cart outside each isolation room (gowns, gloves, masks, eye protection)
  • โ†’N95 or higher respirator with fit-test records
  • โ†’Alcohol-based hand rub at room entry and exit
  • โ†’EHR precaution order and isolation log
  • โ†’Exposure incident report form

Personal protective equipment

  • โ†’Disposable gown
  • โ†’Gloves
  • โ†’Surgical mask or fit-tested respirator
  • โ†’Eye or face protection

5.Procedure

  1. 5.1

    Identify the required precaution type

    Infection Prevention Coordinator

    Review the patient's diagnosis, symptoms and lab results to determine whether standard precautions are sufficient or contact, droplet or airborne precautions are needed, and enter the order in the EHR.

  2. 5.2

    Post signage and prepare the room

    Charge Nurse

    Post the correct precaution sign on the door, stock the PPE cart outside the room, and assign a single room or cohort area if required by the precaution type.

    Checkpoint: Signage matches the active precaution order in the EHR.

  3. 5.3

    Perform hand hygiene before donning

    Clinical Staff Member

    Clean your hands with alcohol-based rub or soap and water immediately before putting on any PPE.

  4. 5.4

    Don PPE in sequence

    Clinical Staff Member

    Put on the gown first, then the mask or respirator, then eye protection, then gloves last, pulling gloves over the gown cuffs.

    Checkpoint: All PPE is fully in place and fitted correctly before entering the room.

  5. 5.5

    Provide care within the room

    Clinical Staff Member

    Limit the number of items brought into the room, keep supplies dedicated to that patient where possible, and avoid touching your face or adjusting PPE with contaminated gloves.

  6. 5.6

    Doff PPE in sequence

    Clinical Staff Member

    Remove gloves first, then perform hand hygiene, remove the gown by rolling it inward, perform hand hygiene again, then remove eye protection and the mask or respirator last, touching only the clean parts.

    Warning: Removing PPE out of sequence, or touching the outside of a contaminated item, can spread the organism to your skin or clothing.

  7. 5.7

    Perform hand hygiene after doffing

    Clinical Staff Member

    Clean your hands immediately after removing the last piece of PPE and again after leaving the room.

  8. 5.8

    Dispose of used PPE

    Clinical Staff Member

    Place used PPE in the designated waste container inside the room, and use a biohazard bag if required by facility policy for the precaution type.

  9. 5.9

    Clean and disinfect the room

    Environmental Services Technician

    Clean and disinfect high-touch surfaces and equipment using a product effective against the identified organism, following the manufacturer's contact time.

  10. 5.10

    Restock the PPE station

    Environmental Services Technician

    Check the PPE cart outside the room during rounds and restock any item that is low or missing before the next shift begins.

    Checkpoint: PPE cart is fully stocked at every shift change.

  11. 5.11

    Report a possible exposure

    Clinical Staff Member

    If skin, eyes or mucous membranes contact blood or body fluid, or PPE fails during care, stop the task, complete the exposure incident report, and notify the supervisor immediately for follow-up.

    Warning: Do not delay exposure reporting; some follow-up treatments are time-sensitive.

  12. 5.12

    Investigate and discontinue precautions

    Infection Prevention Coordinator

    Review exposure reports and any cluster of cases, and discontinue a precaution order only when clinical criteria for discontinuation are met and documented.

6.Quality checks

  • โ†’PPE donning and doffing sequence is observed correctly during audits.
  • โ†’Precaution signage matches the active EHR order on every isolation room.
  • โ†’Isolation rooms pass post-discharge terminal cleaning inspection.
  • โ†’All exposure reports are followed up within the facility's required timeframe.

7.Records

  • โ†’Precaution orders and discontinuation notes in the EHR
  • โ†’PPE competency and respirator fit-test records
  • โ†’Exposure incident reports
  • โ†’Isolation room cleaning logs

8.KPIs

  • โ†’Healthcare-associated infection rate
  • โ†’PPE donning and doffing audit pass rate
  • โ†’Time from exposure to incident report filed
  • โ†’Isolation room turnaround time

9.Common mistakes

  • โ†’Removing gloves and gown together without hand hygiene in between.
  • โ†’Leaving precaution signage up after the order has been discontinued.
  • โ†’Reusing single-use PPE items between patients to save supplies.
  • โ†’Delaying exposure reporting until the end of the shift.

10.Revision history

RevisionDateDescriptionReviewed by
1.0September 15, 2026Initial releaseIlia Pirozhenko

This is a template. Adapt it to your organization, equipment and local regulations before use.

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My glove tore while I was in a patient's room on contact precautions. What should I do?

Stop the task, remove the torn glove and perform hand hygiene, then put on a fresh glove before continuing if there was no skin contact with body fluid. If your skin was exposed, doff all PPE, wash the area, and report it as a possible exposure right away.Source: step 5.11 ยท Report a possible exposure
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